Article

Application of droplet digital PCR to detect human Alu DNA elements in biodistribution assays in pre-clinical studies using rodents

2022-02-10

Abstract excerpt

Biodistribution and engraftment assays for administered human cells in animal organs must be performed to ensure the safety and efficacy of the human cells in preclinical studies. Quantitative real-time polymerase chain reaction (qRT-PCR) is currently the standard method used to detect Alu DNA elements. The recently developed droplet digital PCR (ddPCR) method has been adopted in many biological fields, in which t...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bfc79d1d-93f7-5727-a62a-1af9f453fdaf
DOI: 10.21203/rs.3.rs-1259115/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Application of droplet digital PCR to detect human Alu DNA elements in biodistribution assays in pre-clinical studies using rodentsDOI 10.21203/rs.3.rs-1259115/v1Read

Insights on Droplet Digital PCR–Based Cellular Kinetics and Biodistribution Assay Support for CAR-T Cell TherapySemantic proximity Read

Droplet digital PCR-based testing for donor-derived cell-free DNA in transplanted patients as noninvasive marker of allograft health: Methodological aspectsSemantic proximity Read

Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell productsSemantic proximity Read

Current and Emerging Applications of Droplet Digital PCR in Oncology.Semantic proximity Read

Application of Droplet Digital PCR for the Detection of Vector Copy Number in Clinical CAR/TCR T-Cell ProductsSemantic proximity Read

Digital quantitation of potential therapeutic target RNAs.Semantic proximity Read

Vector copy number analysis using droplet digital PCR method in hematopoietic stem cells and T lymphocytes after lentiviral transduction: optimization and limitationsSemantic proximity Read

Application of droplet digital PCR in minimal residual disease monitoring of rare fusion transcripts and mutations in haematological malignanciesSemantic proximity Read

Droplet digital PCR assay for quantifying of CALR mutant allelic burden in myeloproliferative neoplasmsSemantic proximity Read

Droplet digital PCR or quantitative PCR for in-depth genomic and functional validation of genetically altered rodents.Semantic proximity Read

A Universal Droplet Digital PCR Approach for Monitoring of Graft Health After Transplantation Using a Preselected SNP Set.Semantic proximity Read

Comparison of droplet digital PCR and conventional quantitative PCR for measuring EGFR gene mutationSemantic proximity Read