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Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products

8 May 2020

Abstract excerpt

BACKGROUND: Genetically engineered T cells have become an important therapy for B-cell malignancies. Measuring the efficiency of vector integration into the T cell genome is important for assessing the potency and safety of these cancer immunotherapies. METHODS: A digital droplet polymerase chain reaction (ddPCR) assay was developed and evaluated for assessing the average number of lenti- and retroviral vectors...

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PMID: 32384903
DOI: 10.1186/s12967-020-02358-0

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