An open-source pipeline for calcium imaging and all-optical physiology in human stem cell-derived neurons

Abstract excerpt

High-throughput profiling of neuronal activity at single-cell resolution is essential for advancing our understanding of brain function, enabling large-scale functional screens, and modeling neurological disorders. However, existing approaches are limited by scalability, manual data processing, and variability, thus restricting their ability to detect disease-associated phenotypes. Here, we present a scalable, ope...

Topics

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• Access to Information
• Amyotrophic Lateral Sclerosis
• Drug Discovery
• Drug Evaluation, Preclinical
• Neocortex
• Neural Pathways
• Neurons
• Phenotype

Identifiers and source

Literature Corpus work

4ec4d3f1-3774-5fef-aeca-6bd6e4e6dcea

DOI

10.1101/2025.07.21.664988