Article

MitoToxy Assay: a novel cell-based method for the assessment of metabolic toxicity in a multiwell plate format using a lactate FRET nanosensor, Laconic

2019-03-20

Abstract excerpt

Mitochondrial toxicity is a primary source of pre-clinical drug attrition. Methods that detect mitochondrial toxicity as early as possible during the drug development process are required. Here we introduce a new method for detecting mitochondrial toxicity based on MDA-MB-231 cells stably expressing the genetically-encoded FRET lactate indicator, Laconic. The method takes advantage of the high cytosolic lactate ac...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 48eb3ecb-6a72-50b1-b3d6-9a694410a885
DOI: 10.1101/583096

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

MitoToxy Assay: a novel cell-based method for the assessment of metabolic toxicity in a multiwell plate format using a lactate FRET nanosensor, LaconicDOI 10.1101/583096Read

Research Progress of Lactate and Its Regulated Small Molecule DrugsSemantic proximity Read

Integrative Analysis of Toxicometabolomics and Toxicoproteomics Data: New Molecular Insights into Thiazolidinedione-Induced CardiotoxicitySemantic proximity Read

Oxidative stress, metabolomics profiling, and mechanism of local anesthetic induced cell death in yeastSemantic proximity Read

Phenotype-Based High-Content Screening Using Fluorescent Chemical Bioprobes: Lipid Droplets and Glucose Uptake Quantification in Live Cells.Semantic proximity Read

Plate-Based Assays for the Characterization of Mitochondrial and Cellular Phenotypes.Semantic proximity Read

Label-Free Cell Phenotypic Assays for Assessing Drug Polypharmacology.Semantic proximity Read

Concentration‐dependent metabolic effects of metformin in healthy and Fanconi anemia lymphoblast cellsSemantic proximity Read

Metformin reveals a mitochondrial copper addiction of mesenchymal cancer cellsSemantic proximity Read

Systematic Quantification of Population Cell Death Kinetics in Mammalian CellsSemantic proximity Read

The combination of glycolic acid and D-lactate delays disease progression in SOD-1 ALS mice, partially rescues lethality in iTBPH <sup>pkk(108354)</sup> Drosophila and shows promising results in experimental treatments in two ALS patientsSemantic proximity Read

A Novel Multiple-Read Screen for Metabolically Active Compounds Based on a Genetically Encoded FRET Sensor for ATP.Semantic proximity Read

AgHalo: A Facile Fluorogenic Sensor to Detect Drug-Induced Proteome Stress.Semantic proximity Read