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Subtracting the background: Reducing cell-free DNA’s confounding effects on<i>Mycobacterium tuberculosis</i>quantitation and the sputum microbiome

2024-03-28

Abstract excerpt

Characterising DNA in specimens from people with tuberculosis (TB), a major cause of death, is critical for evaluating diagnostics and the microbiome, yet extracellular DNA, more frequent in people on chemotherapy, confounds results. We evaluated whether nucleic acid dyes [propidium monoazide (PMA), PEMAX] and DNaseI could reduce this. PCR [16S Mycobacterium tuberculosis complex ( Mtb ) qPCR, Xpert MTB/RIF] was do...

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Literature Corpus work
470b84c4-de60-5344-85ec-9c9dc582c5df
DOI
10.1101/2024.03.27.24304911
Open publication

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Subtracting the background: Reducing cell-free DNA’s confounding effects on<i>Mycobacterium tuberculosis</i>quantitation and the sputum microbiomeDOI 10.1101/2024.03.27.24304911
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