Improving clinical significance of PCR: Use of propidium monoazide to distinguish viable from dead <i>staphylococcus aureus</i> and <i>staphylococcus epidermidis</i>

Abstract excerpt

Molecular techniques, such as the polymerase chain reaction (PCR) have high sensitivity when used to diagnose infection, but may detect DNA, RNA, and proteins from dead, as well as viable, bacteria. Propidium monoazide (PMA) is a DNA binding agent, that has the ability to penetrate only dead cells with compromised membranes and has been used in conjunction with real-time PCR to distinguish intact from dead...

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