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A capture methyl-seq protocol with improved efficiency and cost-effectiveness using pre-pooling and enzymatic conversion

2022-10-19

Abstract excerpt

<h4>Objective: </h4> The opportunities for sequencing-based methylome analysis of clinical samples are increasing. To reduce its cost and the amount of genomic DNA required for library preparation, we aimed to establish a capture methyl-seq protocol, which adopts pre-pooling of multiple libraries before hybridization capture and TET2/APOBEC-mediated conversion of unmethylated cytosine to thymine. Results We compa...

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Literature Corpus work
4586a134-d208-5430-9f89-760bf57ebb0c
DOI
10.21203/rs.3.rs-2173809/v1
Open publication

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A capture methyl-seq protocol with improved efficiency and cost-effectiveness using pre-pooling and enzymatic conversionDOI 10.21203/rs.3.rs-2173809/v1
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