Article

Estimation of donor-derived cell-free DNA ratio by SNP capture hybridization and clustering of NGS data

2025-06-12

Abstract excerpt

<title>Abstract</title> <p>Measurement of donor-derived cell-free DNA (dd-cfDNA) enables early, non-invasive monitoring of transplanted organs, including rejection detection. We developed a method to estimate dd-cfDNA ratios by combining capture hybridization of 300 SNPs, next-generation sequencing (NGS), and clustering analysis. Validation was performed using simulated mixtures of fragmented genomic DNA from two...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 44dc1ae9-2247-526d-a681-095c6ee06dfb
DOI: 10.21203/rs.3.rs-6779902/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Estimation of donor-derived cell-free DNA ratio by SNP capture hybridization and clustering of NGS dataDOI 10.21203/rs.3.rs-6779902/v1Read

A SNP-based capture and clustering workflow to assess donor-derived cell-free DNA in transplantation.Semantic proximity Read

An Algorithm Measuring Donor Cell-Free DNA in Plasma of Cellular and Solid Organ Transplant Recipients That Does Not Require Donor or Recipient GenotypingSemantic proximity Read

Validation of a Clinical-Grade Assay to Measure Donor-Derived Cell-Free DNA in Solid Organ Transplant Recipients.Semantic proximity Read

Quantification of transplant-derived circulating cell-free DNA in absence of a donor genotype.Semantic proximity Read

A two-threshold algorithm using donor-derived cell-free DNA fraction and quantity to detect acute rejection after heart transplantationSemantic proximity Read

Cell-free DNA donor fraction analysis in pediatric and adult heart transplant patients by multiplexed allele-specific quantitative PCR: Validation of a rapid and highly sensitive clinical test for stratification of rejection probabilitySemantic proximity Read

Detection of donor-derived cell-free DNA in the setting of multiple kidney transplantationsSemantic proximity Read

Analysis of the primary factors influencing donor derived cell-free DNA testing in kidney transplantation.Semantic proximity Read

Cell-Free DNA in Pediatric Solid Organ Transplantation Using a New Detection Method of Separating Donor-Derived from Recipient Cell-Free DNA.Semantic proximity Read

Comparison of Methods for Donor-Derived Cell-Free DNA Quantification in Plasma and Urine from Solid Organ Transplant RecipientsSemantic proximity Read

A novel donor-derived cell-free DNA assay for the detection of acute rejection in heart transplantationSemantic proximity Read

Multi‐centre analytical performance verification of an IVD assay to quantify donor‐derived cell‐free DNA in solid organ transplant recipientsSemantic proximity Read