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Article

A SNP-based capture and clustering workflow to assess donor-derived cell-free DNA in transplantation.

PloS one - 1 Jan 2026

Mitsunaga Shigeki, Yamada Yohei, Nguyen Phuong Thanh, Fujito Naoko, Nakaoka Hirofumi, Aoyama Hiromichi, Kitamura Hiroshi, Saigo Kenichi, Inoue Ituro, Fujino Akihiro, Shinoda Masahiro, Fukuda Kazumasa, Kitagawa Yuko

Abstract excerpt

Measurement of donor-derived cell-free DNA (dd-cfDNA) enables early, non-invasive monitoring of transplanted organs, including rejection detection. We developed a method to estimate dd-cfDNA ratios using capture hybridization of 300 SNPs, next-generation sequencing (NGS), and clustering analysis. Validation was conducted using simulated mixtures of fragmented genomic DNA from two individuals (0-100%). dd-cfDNA...

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Identifiers

PMID: 41628195
DOI: 10.1371/journal.pone.0342082
PMCID: PMC12863547

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