Article

Optimizing the <i>In Vitro</i> Neuronal Microenvironment to Mitigate Phototoxicity in Live-cell Imaging

2025-08-05

Abstract excerpt

Long-term imaging formats are ideal for capturing dynamic neuronal network formation in vitro, yet fluorescent techniques are often constrained by the impact of phototoxicity on cell survival. Here we present a live-imaging protocol that was optimised via quantitative analysis of 3 target culturing conditions on neuromorphological health: extracellular matrix (human- versus murine-derived laminin), culture media...

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Literature Corpus work: 43722507-45b4-55b2-8134-f00572e2878a
DOI: 10.1101/2025.08.04.668446

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