Article

WS6 enables scalable <i>ex vivo</i> expansion and gene editing of human basal epithelial cells

2025-09-15

Abstract excerpt

Modeling human epithelial diseases and developing cell-based therapies require robust methods to expand and manipulate epithelial stem and progenitor cells in vitro . Basal stem/progenitor cells from stratified epithelia can be expanded in 3T3-J2 fibroblast feeder cell co-culture systems, and the addition of the ROCK inhibitor Y-27632 enhances proliferation and culture longevity, a phenomenon described as ‘condit...

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Literature Corpus work: 41d79c2f-3cd4-59bd-8f4d-2872dd7766e9
DOI: 10.1101/2025.09.15.675861

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