Article

A scalable MNase-seq framework for reproducible nucleosome profiling across pluripotent stem cell and cardiomyocyte models

2026-06-10

Abstract excerpt

<h4>SUMMARY</h4> Micrococcal nuclease (MNase) digestion is widely used to profile chromatin accessibility and nucleosome footprinting. However, its application is often limited by sensitivity to reaction conditions, high cell input requirements, and the lack of standardized protocols across cell types. Here we developed a robust MNase workflow encompassing buffer composition, DNA purification chemistry, fixation...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 41c7aa54-697c-56ee-bcc8-2653b0df8a07
DOI: 10.64898/2026.06.08.731013

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A scalable MNase-seq framework for reproducible nucleosome profiling across pluripotent stem cell and cardiomyocyte modelsDOI 10.64898/2026.06.08.731013Read

High-efficiency genomic mapping of chromatin-associated targets with CUT&RUNSemantic proximity Read

Ultra-parallel ChIP-seq by barcoding of intact nucleiSemantic proximity Read

txci-ATAC-seq, a massive-scale single-cell technique to profile chromatin accessibilitySemantic proximity Read

Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single CellsSemantic proximity Read

Universal NicE-seq for high resolution accessible chromatin profiling for formaldehyde fixed and FFPE tissuesSemantic proximity Read

Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cellsSemantic proximity Read

Emerging Approaches to Profile Accessible Chromatin from Ffpe SectionsSemantic proximity Read

High-Resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci and Alleles.Semantic proximity Read

NucPosDB: a database of nucleosome positioning <i>in vivo</i> and nucleosomics of cell-free DNASemantic proximity Read

High-Quality Nuclei Isolation from Postmortem Human Heart Muscle Tissues for Single-Cell StudiesSemantic proximity Read

Nucleolar endonuclease MBLAC2 orchestrates co-transcriptional ribosomal RNA processing and global transcriptional outputSemantic proximity Read

Whole-genome epigenetic function annotation through sgRNA libraries synthesized by controlled template-dependent elongationSemantic proximity Read