Article

RNA-coupled CRISPR Screens Reveal ZNF207 as a Regulator of LMNA Aberrant Splicing in Progeria

2025-04-27

Abstract excerpt

<h4>SUMMARY</h4> Despite progress in understanding pre-mRNA splicing, the regulatory mechanisms controlling most alternative splicing events remain unclear. We developed CRASP-Seq, a method that integrates pooled CRISPR-based genetic perturbations with deep sequencing of splicing reporters, to quantitively assess the impact of all human genes on alternative splicing from a single RNA sample. CRASP-Seq identifies...

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Literature Corpus work: 40eb8dcf-7396-55be-b329-288b28f68fc9
DOI: 10.1101/2025.04.25.648738

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