Article

A long read optimized <i>de novo</i> transcriptome pipeline reveals novel ocular developmentally regulated gene isoforms and disease targets

2020-08-23

Abstract excerpt

De novo transcriptome construction from short-read RNA-seq is a common method for reconstructing mRNA transcripts within a given sample. However, the precision of this process is unclear as it is difficult to obtain a ground-truth measure of transcript expression. With advances in third generation sequencing, full length transcripts of whole transcriptomes can be accurately sequenced to generate a ground-truth tra...

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Literature Corpus work: 3fdc5848-471c-5dac-8cf2-f207957643bc
DOI: 10.1101/2020.08.21.261644

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