Article

Optimizing mechanostable anchor points of engineered lipocalin in complex with CTLA-4

2021-03-09

Abstract excerpt

We used single-molecule AFM force spectroscopy (AFM-SMFS) to screen residues along the backbone of a non-antibody protein binding scaffold (lipocalin/anticalin), and determine the optimal anchor point that maximizes binding strength of the interaction with its target (CTLA-4). By incorporating non-canonical amino acids into anticalin, and using click chemistry to attach an Fgβ peptide at internal sequence position...

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Literature Corpus work: 336f0fe3-5617-531f-a769-05ed76e61a87
DOI: 10.1101/2021.03.09.434559

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