Article

Mapping proteolytic neo-N termini at the surface of living cells

2020-02-24

Abstract excerpt

N terminomics is a powerful strategy for profiling proteolytic neo-N termini, but its application to cell surface proteolysis has been limited by the low relative abundance of plasma membrane proteins. Here we apply plasma membrane-targeted subtiligase variants to efficiently and specifically capture cell surface N termini in live cells. Using this approach, we sequenced 807 cell surface N termini and quantified c...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3333a093-8d0a-547f-90a0-da06d8f47616
DOI: 10.1101/2020.02.23.961904

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Mapping proteolytic neo-N termini at the surface of living cellsDOI 10.1101/2020.02.23.961904Read

Mapping proteolytic neo-N termini at the surface of living cells.Semantic proximity Read

Exploring the Landscape of Ectodomain Shedding by Quantitative Protein TerminomicsSemantic proximity Read

The carboxy-terminus, a key regulator of protein functionSemantic proximity Read

Protein Termini and Their Modifications Revealed by Positional ProteomicsSemantic proximity Read

N-terminomics identifies widespread endoproteolysis and novel methionine excision in a genome-reduced bacterial pathogenSemantic proximity Read

Comprehensive analysis of human protein N-termini enables assessment of various protein formsSemantic proximity Read

Data processing and analysis in positional proteomicsSemantic proximity Read

Identification of Human Host Substrates of the SARS-CoV-2 Mpro and PLpro Using Subtiligase N-TerminomicsSemantic proximity Read

EndoNB: A general strategy to study the internalization of cell surface proteinsSemantic proximity Read

Protein C-terminal variations impact proteostasis.Semantic proximity Read

Development of a New N-Terminomic Method to Study the Pathodegradome of the <i>Staphylococcus aureus</i> V8 Protease in Human NeutrophilsSemantic proximity Read

N-Terminal Modification of Proteins with Subtiligase Specificity Variants.Semantic proximity Read