Article
Mapping proteolytic neo-N termini at the surface of living cells.
Proceedings of the National Academy of Sciences of the United States of America - 23 Feb 2021
Weeks Amy M, Byrnes James R, Lui Irene, Wells James A
Abstract excerpt
N terminomics is a powerful strategy for profiling proteolytic neo-N termini, but its application to cell surface proteolysis has been limited by the low relative abundance of plasma membrane proteins. Here we apply plasma membrane-targeted subtiligase variants (subtiligase-TM) to efficiently and specifically capture cell surface N termini in live cells. Using this approach, we sequenced 807 cell surface N...
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