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Harnessing Non-standard Nucleic Acids for Highly Sensitive Icosaplex (20-Plex) Detection of Microbial Threats for Environmental Surveillance

3 Feb 2025

Abstract excerpt

Environmental surveillance and clinical diagnostics heavily rely on the polymerase chain reaction (PCR) for target detection. A growing list of microbial threats warrants new PCR-based detection methods that are highly sensitive, specific, and multiplexable. Here, we introduce a PCR-based icosaplex (20-plex) assay for detecting 18 enteropathogen and two antimicrobial resistance genes. This multiplexed PCR assay...

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Share to Advanced biosensing and bioanalysis techniques
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Share to Viral gastroenteritis research and epidemiology

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PMID: 39898969
DOI: 10.1021/acssynbio.4c00619

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