Article

E2-Regulated Transcriptome Complexity Revealed by Long-Read Direct RNA Sequencing: From Isoform Discovery to Truncated Proteins

2025-08-02

Abstract excerpt

Estrogen receptor alpha (ERα)-positive (ER+) breast cancers are driven by 17β-estradiol (E2) binding to ERα, which transcriptionally regulates downstream target genes. Although microarrays and conventional RNA sequencing have identified E2 target genes, pre-designed probes and short read lengths are limited in accurately capturing complex transcript structures. Long-read RNA sequencing offers a solution by spannin...

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Literature Corpus work: 3010fb45-7c47-5cc6-a147-4f52fba50607
DOI: 10.1101/2025.07.31.667732

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