Article

CRISPR-Targeted CAR Gene Insertion Using Cas9/RNP and AAV6 Enhances Anti-AML Activity of Primary NK Cells

2021-03-17

Abstract excerpt

Human peripheral blood natural killer (NK) cells have intense antitumor activity and have been used successfully in several clinical trials. Modifying NK cells with a chimeric antigen receptor (CAR) can improve their targeting and increase specificity. Recently, we described an efficient method for gene targeting in NK cells using Cas9/ribonucleoprotein (RNP) complexes. Here we combined this approach with single-s...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 916e7275-e01b-58ba-aafa-85d30e777336
DOI: 10.1101/2021.03.17.435886

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Targeted CAR Gene Insertion Using Cas9/RNP and AAV6 Enhances Anti-AML Activity of Primary NK CellsDOI 10.1101/2021.03.17.435886Read

Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAVSemantic proximity Read

Highly efficient site-directed gene insertion in primary human natural killer cells using homologous recombination and CRISPaint delivered by AAVSemantic proximity Read

A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer ImmunotherapySemantic proximity Read

Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor functionSemantic proximity Read

A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer ImmunotherapySemantic proximity Read

Nonviral technologies can pave the way for CAR-NK cell therapySemantic proximity Read

CRISPR, CAR-T, and NK: Current applications and future perspectivesSemantic proximity Read

Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 RibonucleoproteinsSemantic proximity Read

A robust platform for expansion and genome editing of primary human natural killer cellsSemantic proximity Read

CRISPR Gene Editing of Human Primary NK and T Cells for Cancer ImmunotherapySemantic proximity Read

Drug target validation in primary human natural killer cells using CRISPR RNPSemantic proximity Read

Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potencySemantic proximity Read