Article

A novel genotyping method to determine copy number in a mouse line commonly used for inducible transgene expression in brain and spinal cord

2022-02-10

Abstract excerpt

<h4>Background: </h4> : The NEFH-tTA mouse has the human neurofilament heavy polypeptide promoter directing tetracycline-controlled transactivator protein (tTa) expression to the brain and spinal cord, allowing tissue-specific and doxycycline-suppressible expression of a target gene. Current genotyping protocols can only differentiate between wild-type and transgenic animals. Being able to differentiate between h...

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Literature Corpus work: 20228580-87ec-542c-b4d8-2d46684631b0
DOI: 10.12688/f1000research.26550.2

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