Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A simple PCR method for rapid genotype analysis of the TH-MYCN transgenic mouse.

PloS one - 4 Sept 2009

Haraguchi Seiki, Nakagawara Akira

Abstract excerpt

BACKGROUND: The TH-MYCN transgenic mouse is the most widely used murine model of human neuroblastoma, in which a human MYCN oncogene is targeted to neuroectodermal cells of developing mice under the influence of the rat tyrosine hydroxylase promoter. So far, homozygous transgenic mice have been identified by either Southern blot or quantitative real-time PCR. PRINCIPAL FINDINGS: To establish a simple and reliable...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blotting, Southern
Share to Crosses, Genetic
Share to Genetic Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mice, Transgenic

Share to Neuroblastoma

Share to Proto-Oncogene Proteins c-myc

Share to Transgenes

Share to Tyrosine 3-Monooxygenase

Source

Open in PubMed Open full text

Identifiers

PMID: 19730731
DOI: 10.1371/journal.pone.0006902
PMCID: PMC2731925

Genes

MYCN

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A simple PCR method for rapid genotype analysis of the TH-MYCN transgenic mouse.PMID 19730731Read ↗

Tumor Development, Growth Characteristics and Spectrum of Genetic Aberrations in the TH-MYCN Mouse Model of NeuroblastomaSemantic proximity Read ↗

A novel genotyping method to determine copy number in a mouse line commonly used for inducible transgene expression in brain and spinal cordSemantic proximity Read ↗

A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studiesSemantic proximity Read ↗

Localization of transgenes and genotyping of H-2kb-tsA58 transgenic mice.Semantic proximity Read ↗

A protocol for locating and counting transgenic sequences from laboratory animals using a map-then-capture (MapCap) sequencing workflow: procedure and application of resultsSemantic proximity Read ↗

Rapid and precise genotyping of transgene zygosity in mice using an allele-specific method.Semantic proximity Read ↗

One-hour universal protocol for mouse genotyping.Semantic proximity Read ↗

miRNA Expression Profiling of the Murine TH-MYCN Neuroblastoma Model Reveals Similarities with Human Tumors and Identifies Novel Candidate MiRNAsSemantic proximity Read ↗

Expression of N-myc andMRP genes and their relationship to N-myc gene dosage and tumor formation in a murine neuroblastoma modelSemantic proximity Read ↗

Cell lines from MYCN transgenic murine tumours reflect the molecular and biological characteristics of human neuroblastomaSemantic proximity Read ↗

Genetically engineered murine models – Contribution to our understanding of the genetics, molecular pathology and therapeutic targeting of neuroblastomaSemantic proximity Read ↗

Histological profile of tumours from <i>MYCN</i> transgenic miceSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.