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Article

In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs.

RNA (New York, N.Y.) - 1 Jun 2012

Gurtan Allan M, Lu Victoria, Bhutkar Arjun, Sharp Phillip A

Abstract excerpt

Dicer is an RNase III family endoribonuclease and haploinsufficient tumor suppressor that processes mature miRNAs from the 5' (5p) or 3' (3p) arm of hairpin precursors. In murine Dicer knockout fibroblasts, we expressed human Dicer with point mutations in the RNase III, helicase, and PAZ domains and characterized miRNA expression by Northern blot and massively parallel sequencing of small RNAs. We report that...

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Identifiers

PMID: 22546613
DOI: 10.1261/rna.032680.112
PMCID: PMC3358635

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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