Article

The effect of reverse transcription enzymes and conditions on high throughput amplicon sequencing of the 16S rRNA

2019-07-15

Abstract excerpt

It is assumed that the sequencing of ribosomes better reflects the active microbial community than the sequencing of the ribosomal RNA encoding genes. Yet, many studies exploring microbial communities in various environments, ranging from the human gut to deep oceans, questioned the validity of this paradigm due to the discrepancies between the DNA and RNA based communities. Here we focus on an often neglected key...

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Literature Corpus work: 1b48263c-450d-521e-a9f8-72d15d260d20
DOI: 10.7287/peerj.preprints.27780v2

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