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Article

Post-amplification Klenow fragment treatment alleviates PCR bias caused by partially single-stranded amplicons.

Journal of microbiological methods - 1 Apr 2005

Egert Markus, Friedrich Michael W

Abstract excerpt

Partially single-stranded amplicons, formed during PCR amplification of single and mixed templates, are a potential source of bias in genetic diversity studies. The analysis of 16S rRNA gene diversity in mixed template samples by the fingerprinting technique terminal restriction fragment length polymorphism (T-RFLP) analysis can be biased by the occurrence of pseudo-T-RFs, i.e., restriction fragments occurring in...

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Identifiers

PMID: 15676197
DOI: 10.1016/j.mimet.2004.11.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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