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Use of rpoB and 16S rRNA genes to analyse bacterial diversity of a tropical soil using PCR and DGGE.

Letters in applied microbiology - 1 Jan 2002

Peixoto R S, da Costa Coutinho H L, Rumjanek N G, Macrae A, Rosado A S

Abstract excerpt

AIM: To evaluate the rpoB gene as a biomarker for PCR-DGGE microbial analyses using soil DNA from the Cerrado, Brazil. METHODS: DNA extraction from soil was followed by Polymerase Chain Reaction (PCR) amplification of rpoB and 16S rRNA genes. PCR products were compared by Denaturing Gradient Gel Electrophoresis (DGGE) to compare gene/community profiles. RESULTS: The rpoB DGGE profiles comprised fewer bands than...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 12358695
DOI: 10.1046/j.1472-765x.2002.01183.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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