Back to search

Article

A simple, cost-effective and automation-friendly direct PCR approach for bacterial community analysis

2021-03-02

Abstract excerpt

<h4>ABSTRACT</h4> Bacterial communities in water, soil, and humans play an essential role in environmental ecology and human health. PCR-based amplicon analysis, such as 16S ribosomal RNA sequencing, is a fundamental tool for quantifying and studying microbial composition, dynamics, and interactions. However, given the complexity of microbial communities, a substantial number of samples becomes necessary to analy...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
b73257e8-8cae-5193-b69a-63705de22183
DOI
10.1101/2021.03.01.433496
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
A simple, cost-effective and automation-friendly direct PCR approach for bacterial community analysisDOI 10.1101/2021.03.01.433496
A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communitiesSemantic proximity
Harnessing the Power of PCR Molecular Fingerprinting Methods and Next Generation Sequencing for Understanding Structure and Function in Microbial Communities.Semantic proximity
Optimisation of a DNA extraction protocol for improving the bacterial and fungal classification based on Nanopore sequencingSemantic proximity
Improved DNA Extraction and Amplification Strategy for 16S rRNA Gene Amplicon-Based Microbiome StudiesSemantic proximity
A Comparison of Three Automated Nucleic Acid Extraction Systems for Human Stool SamplesSemantic proximity
Optimisation of a DNA extraction protocol for improving the bacterial and fungal classification based on Nanopore sequencingSemantic proximity
Portable In-Field DNA Sequencing for Rapid Detection of Pathogens and Antimicrobial Resistance: A Proof-of-Concept StudySemantic proximity
Portable In-Field DNA Sequencing for Rapid Detection of Pathogens and Antimicrobial Resistance: A Proof-of-Concept StudySemantic proximity
A rapid, inexpensive, culture-free, universal bacterial identification system using internal transcribed spacer targeting primers: a proof-of-principle studySemantic proximity
Speed, accuracy, sensitivity and quality control choices for detecting clinically relevant microbes in whole blood from patientsSemantic proximity
Impact of DNA polymerase choice on assessment of bacterial communities by a <i>Legionella</i> genus-specific next-generation sequencing approachSemantic proximity
Toward standards in clinical microbiome studies: comparison of three DNA extraction methods and two bioinformatic pipelinesSemantic proximity
Select a neighboring publication to make it the new centre.