Article

Sustained exposure to trypsin causes cells to transition into a state of reversible stemness that is amenable to transdifferentiation

2019-06-23

Abstract excerpt

<h4>ABSTRACT</h4> During cell culture, trypsin, a serine protease, is applied to cells for 5-10 minutes to separate them from each other and from the underlying substratum so that they can be transferred to a different vessel, for re-plating, after growth medium containing 10 % serum has been added to the cells, in a well-known technique known as ‘passaging’. The serum in the growth medium contains alpha-1 antitr...

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Identifiers and source

Literature Corpus work: 18520dc1-0e46-5549-86d9-eb4e9dfd58e8
DOI: 10.1101/679928

Open publication

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