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Article

Use of a Collagen Membrane to Enhance the Survival of Primary Intestinal Epithelial Cells.

Journal of cellular physiology - 1 Sept 2017

Di Claudio Fiorella, Muglia Cecilia I, Smaldini Paola L, Orsini Delgado María Lucía, Trejo Fernando M, Grigera J Raúl, Docena Guillermo H

Abstract excerpt

Intestinal epithelial cell culture is important for biological, functional, and immunological studies. Since enterocytes have a short in vivo life span due to anoikis, we aimed to establish a novel and reproducible method to prolong the survival of mouse and human cells. Cells were isolated following a standard procedure, and cultured on ordered-cow's collagen membranes. A prolonged cell life span was achieved;...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27626762
DOI: 10.1002/jcp.25594

Genes

TLR4
TLR5
TNF
TSLP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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