Article

CRISPR screening of porcine sgRNA library identified host factors essential for Japanese encephalitis virus replication

2019-11-13

Abstract excerpt

<h4>ABSTRACT</h4> Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic flavivirus that causes encephalitis and reproductive disorders in mammalian species. However, key host genes involved in the JEV life cycle and cell death are poorly understood. Here, we designed 85,674 single guide RNAs (sgRNAs) targeting 17,743 protein-coding genes, 11,053 long ncRNAs (lncRNAs), and 551 microRNAs (miRNAs) in the po...

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Literature Corpus work: 17e4bfb0-ff00-5669-a1a5-4ea8367fd86b
DOI: 10.1101/840835

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