Back to search

Article

Similar but distinct: the impact of biomechanical forces and culture age on the production, cargo loading, and biological efficacy of human megakaryocytic extracellular vesicles for applications in cell and gene therapies

2023-04-29

Abstract excerpt

Megakaryocytic extracellular vesicles (MkEVs) promote the growth and megakaryopoiesis of hematopoietic stem and progenitor cells (HSPCs) largely through endogenous miR-486-5p and miR-22-3p cargo. Here, we examine the impact of biomechanical force and culture age/differentiation on the formation, properties, and biological efficacy of MkEVs. We applied biomechanical force to Mks using two methods: shake flask cultu...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
17baf88a-be3f-590d-8886-88b7e35d1d7a
DOI
10.1101/2023.04.26.538401
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Similar but distinct: the impact of biomechanical forces and culture age on the production, cargo loading, and biological efficacy of human megakaryocytic extracellular vesicles for applications in cell and gene therapiesDOI 10.1101/2023.04.26.538401
Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profilingSemantic proximity
Baffled‐flow culture system enables the mass production of megakaryocytes from human embryonic stem cells by enhancing mitochondrial functionSemantic proximity
Dynamic Mechanical Loading Reprograms Meniscus Cell-Derived Extracellular Vesicles to Enhance Their Regenerative PotencySemantic proximity
Scale-out production of extracellular vesicles derived from natural killer cells via mechanical stimulation in a seesaw-motion bioreactor for cancer therapySemantic proximity
Arp2/3 mediated dynamic lamellipodia of the hPSC colony edges promote liposome-based DNA deliverySemantic proximity
Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle productionSemantic proximity
Matrix Nanoscale Mechanics Regulates Exosome Production by Mesenchymal Stem CellsSemantic proximity
Extracellular Vesicle-Mediated Modulation of Stem-like Phenotype in Breast Cancer Cells under Fluid Shear Stress.Semantic proximity
Native and engineered human megakaryocytic extracellular vesicles for targeted non-viral cargo delivery to blood stem cellsSemantic proximity
Small extracellular vesicles reflect senescence progression in human bone marrow–derived mesenchymal stem cells during hollow fiber bioreactor cultureSemantic proximity
Growth Factor-Based Manufacturing of Human Pluripotent Stem Cell-Derived Cardiomyocytes Using the Vertical Wheel Bioreactor SystemSemantic proximity
Small-scale bioreactor cultivation of HEK293-based suspension cells increases extracellular vesicle yieldSemantic proximity
Select a neighboring publication to make it the new centre.