Article

Overcoming Protein A-driven Nonspecific Antibody Staining of <i>S. aureus</i> in Immunofluorescence Microscopy

2026-03-26

Abstract excerpt

The ability to detect host cell factors during Staphylococcus aureus infection in vitro by immunofluorescence microscopy is severely hampered by staphylococcal protein A (SpA), a cell wall-anchored protein that binds the fragment crystallizable (Fc) region of immunoglobulins. This interaction generates strong nonspecific fluorescent signals on the bacterial surface, complicating data interpretation and limiting...

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Literature Corpus work: 15918b21-bef1-5e6a-91f8-050b16286b6a
DOI: 10.64898/2026.03.26.713373

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