Article

Detection of isoforms and genomic alterations by high-throughput full-length single-cell RNA sequencing in ovarian cancer

2022-12-14

Abstract excerpt

Understanding the complex background of cancer requires genotype-phenotype information in single-cell resolution. Here, we perform long-read single-cell RNA sequencing (scRNA-seq) on clinical samples from three ovarian cancer patients presenting with omental metastasis and increase the PacBio sequencing depth to 12,000 reads per cell. Our approach captures 152,000 isoforms, of which over 52,000 are novel. Isoform-...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 31c6912d-3529-5af4-b7c3-5ae89b7156f1
DOI: 10.1101/2022.12.12.520051

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Detection of isoforms and genomic alterations by high-throughput full-length single-cell RNA sequencing in ovarian cancerDOI 10.1101/2022.12.12.520051Read

Comprehensive long-read transcriptome analysis uncovers alternative RNA processing feature and isoform diversity in ovarian cancer progressionSemantic proximity Read

Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer.Semantic proximity Read

An isoform-resolution transcriptomic atlas of colorectal cancer from long-read single-cell sequencingSemantic proximity Read

Exploring the tumor micro-environment in ovarian cancer histotypes and tumor sitesSemantic proximity Read

Single-Cell RNA Sequencing Reveals the Tissue Architecture in Human High-Grade Serous Ovarian CancerSemantic proximity Read

Single-Cell Transcriptomics Reveals Tissue Architecture in Ovarian CarcinosarcomaSemantic proximity Read

Redefining colorectal cancer classification and clinical stratification through a single-cell atlasSemantic proximity Read

Single-cell and bulk transcriptome sequencing identifies two epithelial tumor cell states and refines the consensus molecular classification of colorectal cancerSemantic proximity Read

Targeted Transcriptome Analysis using Synthetic Long Read Sequencing Uncovers Isoform Reprograming in the Progression of Colon CancerSemantic proximity Read

Genetic characterization of primary and metastatic high-grade serous ovarian cancer tumors reveals distinct features associated with survivalSemantic proximity Read

Single-cell RNA sequencing in ovarian cancer: revealing new perspectives in the tumor microenvironmentSemantic proximity Read

Understanding the cellular and molecular heterogeneity in colorectal cancer through the use of single-cell RNA sequencingSemantic proximity Read