Article

Signature tagged mutagenesis of Haemophilus influenzae

2001-01-01

Abstract excerpt

Signature Tagged Mutagenesis (STM) is a validated means of creating and labelling transposon generated bacterial mutants that can be screened in vivo for loss of virulence. The pathogenic H. influenzae strain Rd-b+ was mutagenized with Tn<i>1545</i>-Ʌ3 and a variety of stable single gene disruption mutants were created. Tu<i>1545</i>-Ʌ3 was adapted to contain signature tags, and transposons that contained pre-sele...

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Literature Corpus work: 0c78d952-d7f0-53a2-bdac-f43ef9d1cb53
DOI: 10.21954/ou.ro.0000f994

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