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Article

Essential genes in the infection model of Pseudomonas aeruginosa-PCR-based signature-tagged mutagenesis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Kukavica-Ibrulj Irena, Levesque Roger C

Abstract excerpt

PCR-based signature tagged mutagenesis is an "en masse" screening technique based upon unique oligonucleotide tags (molecular barcodes) for identification of genes that will diminish or enhance maintenance of an organism in a specific ecological niche or environment. PCR-based STM applied to Pseudomonas aeruginosa permitted the identification of genes essential for in vivo maintenance by transposon insertion and...

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PMID: 25636615
DOI: 10.1007/978-1-4939-2398-4_7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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