In vivo transposon mutagenesis in Haemophilus influenzae.

doi:10.1128/AEM.64.12.4697-4702.1998

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Article

In vivo transposon mutagenesis in Haemophilus influenzae.

Applied and environmental microbiology - 1 Dec 1998

Kraiss A, Schlör S, Reidl J

Abstract excerpt

In order to devise an in vivo insertion mutagenesis scheme for Haemophilus influenzae, a novel set of transposons has been constructed. These are Tn10-based minitransposons carried on pACYC184- and pACYC177-based replicons, which are suitable for in vivo transposition in H. influenzae. The transp...

Topics

Chloramphenicol O-Acetyltransferase
DNA Transposable Elements
DNA, Bacterial
Genotype
Haemophilus influenzae
Humans
Mutagenesis, Insertional
Plasmids
Polymerase Chain Reaction
Recombinant Fusion Proteins
Replicon
beta-Galactosidase

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