Article
In vivo transposon mutagenesis in Haemophilus influenzae.
Applied and environmental microbiology - 1 Dec 1998
Kraiss A, Schlör S, Reidl J
Abstract excerpt
In order to devise an in vivo insertion mutagenesis scheme for Haemophilus influenzae, a novel set of transposons has been constructed. These are Tn10-based minitransposons carried on pACYC184- and pACYC177-based replicons, which are suitable for in vivo transposition in H. influenzae. The transposon delivery system was designed to contain an H. influenzae-specific uptake signal sequence which facilitates DNA...
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