Article
Aerobic growth deficient Haemophilus influenzae mutants are non-virulent: implications on metabolism.
International journal of medical microbiology : IJMM - 1 Jun 2003
Herbert Mark, Kraiss Anita, Hilpert Anna-Karina, Schlör Stefan, Reidl Joachim
Abstract excerpt
We investigated aerobic metabolism in Haemophilus influenzae to better understand its essential physiological growth pathways. We describe the isolation and characterization of transposon insertions leading to knockout mutations in lpdA, encoding dihydrolipoamide dehydrogenase. H. influenzae Rd lpdA::Tn10d-cat mutants were unable to grow aerobically and an H. influenzae type b lpdA::Tn10d-cat mutant was...
Topics
- Aerobiosis
- Animals
- Citric Acid Cycle
- DNA Transposable Elements
- DNA, Bacterial
- Gene Deletion
- Haemophilus Infections
- Haemophilus influenzae
- Ketoglutarate Dehydrogenase Complex
- Mutagenesis, Insertional
- Mutation
- Pyruvate Dehydrogenase (Lipoamide)
- Rats
- Rats, Sprague-Dawley