Article

Incorporation of TRDV segments into TCR alpha chains

2023-02-27

Abstract excerpt

In human tumor models we tried to identify and clone the TCR of tumor-reactive T cells enriched in mixed lymphocyte tumor-cell cultures (MLTC). In a particular MLTC, we identified a predominant TCR beta chain using the Beta Mark Vbeta Kit, but a corresponding alpha chain could not be amplified via RT-PCR using TRAV -specific forward primers. Therefore, we applied 5’RACE to obtain the TCR alpha chain sequences. Th...

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Literature Corpus work: 0ab09353-fa64-541c-88b0-2c6fee481364
DOI: 10.1101/2023.02.26.530072

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