Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Double-step and inverse polymerase chain reaction for sensitive detection and cloning of T cell receptor variable region sequences.

International immunology - 1 Oct 1991

Inaba T, Koseki H, Suzuki M, Taniguchi M

Abstract excerpt

We have developed a sensitive and rapid method for detection and cloning of cDNA amplified by double-step and inverse polymerase chain reaction (PCR) techniques. The blunt-ended double-strand cDNA libraries were circularized with T4 ligase and subsequently amplified by double-step PCR with two sets of primers of outward orientation (at amounts of 1-10 pmol in the first step and 100 pmol in the second step) which...

Topics

Animals
Base Sequence
Cloning, Molecular
Gene Expression
Gene Library
Genetic Variation
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger
Receptors, Antigen, T-Cell, alpha-beta

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sensitivity and Specificity

Source

Identifiers

PMID: 1836737
DOI: 10.1093/intimm/3.10.1053

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1836737-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Double-step and inverse polymerase chain reaction for sensitive detection and cloning of T cell receptor variable region sequences.PMID 1836737Read ↗

Systematic Comparative Evaluation of Methods for Investigating the TCRβ RepertoireSemantic proximity Read ↗

Structure and diversity of T-lymphocyte antigen receptors alpha and gamma in Xenopus.Semantic proximity Read ↗

An improved methodology to detect human T cell receptor beta variable family gene expression patterns.Semantic proximity Read ↗

Identification of TRDV-TRAJ V domains in human and mouse T-cell receptor repertoiresSemantic proximity Read ↗

Analysis of rearranged T-cell receptor beta-chain genes by polymerase chain reaction (PCR) DNA sequencing and automated high resolution PCR fragment analysisSemantic proximity Read ↗

Analysis of diversity of T cell antigen receptor genes using polymerase chain reaction and sequencing gel electrophoresis.Semantic proximity Read ↗

Systematic design and testing of nested (RT-)PCR primers for specific amplification of mouse rearranged/expressed immunoglobulin variable region genes from small number of B cellsSemantic proximity Read ↗

Quantitative analysis of the usage of human T-cell receptor alpha and beta chain variable regions by reverse dot-blot hybridization.Semantic proximity Read ↗

Organization, sequence, and function of 34.5 kb of genomic DNA encompassing several murine T-cell receptor alpha/delta variable gene segments.Semantic proximity Read ↗

Incorporation of TRDV segments into TCR alpha chainsSemantic proximity Read ↗

The application of real-time PCR to the analysis of T cell repertoires.Semantic proximity Read ↗

A new method for quantitative analysis of the mouse T-cell receptor V region repertoires: comparison of repertoires among strains.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.