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Article

Double-step and inverse polymerase chain reaction for sensitive detection and cloning of T cell receptor variable region sequences.

International immunology - 1 Oct 1991

Inaba T, Koseki H, Suzuki M, Taniguchi M

Abstract excerpt

We have developed a sensitive and rapid method for detection and cloning of cDNA amplified by double-step and inverse polymerase chain reaction (PCR) techniques. The blunt-ended double-strand cDNA libraries were circularized with T4 ligase and subsequently amplified by double-step PCR with two sets of primers of outward orientation (at amounts of 1-10 pmol in the first step and 100 pmol in the second step) which...

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PMID: 1836737
DOI: 10.1093/intimm/3.10.1053

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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