Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Replicate sequencing libraries are important for quantification of allelic imbalance.

Nature communications - 7 Jun 2021

Mendelevich Asia, Vinogradova Svetlana, Gupta Saumya, Mironov Andrey A, Sunyaev Shamil R, Gimelbrant Alexander A

Abstract excerpt

A sensitive approach to quantitative analysis of transcriptional regulation in diploid organisms is analysis of allelic imbalance (AI) in RNA sequencing (RNA-seq) data. A near-universal practice in such studies is to prepare and sequence only one library per RNA sample. We present theoretical and experimental evidence that data from a single RNA-seq library is insufficient for reliable quantification of the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Algorithms
Share to Allelic Imbalance

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Library

Share to Mice, 129 Strain

Share to Models, Genetic

Share to Sequence Analysis, RNA

Share to Transcriptome

Source

Open in PubMed Open full text

Identifiers

PMID: 34099647
DOI: 10.1038/s41467-021-23544-8
PMCID: PMC8184992

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Replicate sequencing libraries are important for quantification of allelic imbalance.PMID 34099647Read ↗

Airpart: interpretable statistical models for analyzing allelic imbalance in single-cell datasets.Semantic proximity Read ↗

Power calculator for detecting allelic imbalance using hierarchical Bayesian model.Semantic proximity Read ↗

Key considerations for measuring allelic expression on a genomic scale using high-throughput sequencing.Semantic proximity Read ↗

A compendium of methods: Searching allele specific expression via RNA sequencing.Semantic proximity Read ↗

Computational methods for allele-specific expression in single cells.Semantic proximity Read ↗

Tools and best practices for data processing in allelic expression analysis.Semantic proximity Read ↗

Detecting cell-type-specific allelic expression imbalance by integrative analysis of bulk and single-cell RNA sequencing data.Semantic proximity Read ↗

Detecting cell-type-specific allelic expression imbalance by integrative analysis of bulk and single-cell RNA sequencing dataSemantic proximity Read ↗

The promise of long-read RNA-seq: reducing bias in analyses of allele imbalance.Semantic proximity Read ↗

AllelicImbalance: an R/bioconductor package for detecting, managing, and visualizing allele expression imbalance data from RNA sequencing.Semantic proximity Read ↗

Identification of imprinted loci by transcriptome sequencing.Semantic proximity Read ↗

Statistical inference of allelic imbalance from transcriptome data.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.