Article

High resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-seq)

2018-11-05

Abstract excerpt

Two-hybrid systems test for protein-protein interactions and can provide important information for genes with unknown function. Despite their success, two-hybrid systems have remained mostly untouched by improvements from next-generation DNA sequencing. Here we present a method for all-versus-all protein interaction mapping (AVA-seq) that utilizes next-generation sequencing to remove multiple bottlenecks of the tw...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0946a7d3-dfc1-5003-b98f-52826d1e5f85
DOI: 10.1101/462309

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

High resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-seq)DOI 10.1101/462309Read

Long-read sequencing to detect full-length protein-protein interactionsSemantic proximity Read

The split-hybrid system. Uncoding multiprotein networks and defining mutations that affect protein interactions.Semantic proximity Read

Multiplex mapping of protein-protein interaction interfacesSemantic proximity Read

Interactome: gateway into systems biology.Semantic proximity Read

The social architecture of an in-depth cellular protein interactomeSemantic proximity Read

In silico two-hybrid system for the selection of physically interacting protein pairs.Semantic proximity Read

Understanding gene and allele function with two-hybrid methods.Semantic proximity Read

High-accuracy mapping of human and viral direct physical protein-protein interactions using the novel computational system AlphaFold-pairsSemantic proximity Read

Protein-protein interactions: the yeast two-hybrid system.Semantic proximity Read

Transposon-Display of AI-designed binders enables manipulation of the proteome in human cellsSemantic proximity Read

Pooled PPIseq: screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platformSemantic proximity Read

Combining DNA and protein alignments to improve genome annotation with LiftOnSemantic proximity Read