Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.

doi:10.1016/0378-1097(91)90324-4

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Article

Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.

FEMS microbiology letters - 1 Aug 1991

Fujimura H

Abstract excerpt

For the transformation of the yeast Saccharomyces kluyveri, ura3 mutants were obtained by 5-fluoro-orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae-based plasmids. In...

Topics

Ampicillin
Ampicillin Resistance
Centromere
Electrophoresis, Agar Gel
Ethyl Methanesulfonate
Mutation
Orotic Acid
Plasmids
Saccharomyces
Saccharomyces cerevisiae
Transformation, Genetic
Uracil

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