Article
Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.
FEMS microbiology letters - 1 Aug 1991
Fujimura H
Abstract excerpt
For the transformation of the yeast Saccharomyces kluyveri, ura3 mutants were obtained by 5-fluoro-orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae-based plasmids. In the transformed cells, a S. cerevisiae centromere-based plasmid was stably replicated autonomously. Thus, this system...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
