Article
The small Mr Ras-like GTPase Rap1 and the phospholipase C pathway act to regulate phagocytosis in Dictyostelium discoideum.
Molecular biology of the cell - 1 Feb 1999
Seastone D J, Zhang L, Buczynski G, Rebstein P, Weeks G, Spiegelman G, Cardelli J
Abstract excerpt
The function of the small-Mr Ras-like GTPase Rap1 remains largely unknown, but this protein has been demonstrated to regulate cortical actin-based morphologic changes in Dictyostelium and the oxidative burst in mammalian neutrophils. To test whether Rap1 regulates phagocytosis, we biochemically analyzed cell lines that conditionally and modestly overexpressed wild-type [Rap1 WT(+)], constitutively active [Rap1...
Topics
- Animals
- Calcium
- Dictyostelium
- Diglycerides
- Endosomes
- Enzyme Inhibitors
- Estrenes
- GTP-Binding Proteins
- Gene Expression
- Genes, Protozoan
- Lysosomes
- Mutation
- Naphthalenes
- Phagocytosis