Article
Protein glycosylation mutants of procyclic Trypanosoma brucei: defects in the asparagine-glycosylation pathway.
Glycobiology - 1 Feb 1999
Hwa K Y, Acosta-Serrano A, Khoo K H, Pearson T, Englund P T
Abstract excerpt
We employed a genetic approach to study protein glycosylation in the procyclic form of the parasite Trypanosoma brucei. Two different mutant parasites, ConA 1-1 and ConA 4-1, were isolated from mutagenized cultures by selecting cells which resisted killing or agglutination by concanavalin A. Both...
Topics
- Animals
- Asparagine
- Carbohydrate Sequence
- Concanavalin A
- Drug Resistance
- Flagella
- Glycosylation
- Membrane Glycoproteins
- Molecular Sequence Data
- Mutation
- Protein Processing, Post-Translational
- Protozoan Proteins
- Spectrometry, Mass, Fast Atom Bombardment
- Trypanosoma brucei brucei