Article
Depletion of Escherichia coli 4.5S RNA leads to an increase in the amount of protein elongation factor EF-G associated with ribosomes.
European journal of biochemistry - 1 Jan 1999
Nakamura K, Fujii Y, Shibata T, Yamane K
Abstract excerpt
In Escherichia coli, 4.5S RNA is found in complexes with both protein translocation protein, Ffh (a bacterial homolog of mammalian SRP54) and protein synthesis elongation factor G (EF-G). To analyze the function of 4.5S RNA in translation, we initially assessed the sensitivity of the association...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Binding, Competitive
- Centrifugation, Density Gradient
- Conserved Sequence
- Escherichia coli
- Fusidic Acid
- Guanosine Diphosphate
- Mutation
- Nucleic Acid Conformation
- Peptide Elongation Factor G
- Peptide Elongation Factors
- Protein Biosynthesis
- Puromycin
- RNA, Bacterial
- RNA, Ribosomal, 23S
- Ribosomes