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Article

Requirement for transient metal ions revealed through computational analysis for DNA polymerase going in reverse.

Proceedings of the National Academy of Sciences of the United States of America - 22 Sept 2015

Perera Lalith, Freudenthal Bret D, Beard William A, Shock David D, Pedersen Lee G, Wilson Samuel H

Abstract excerpt

DNA polymerases facilitate faithful insertion of nucleotides, a central reaction occurring during DNA replication and repair. DNA synthesis (forward reaction) is "balanced," as dictated by the chemical equilibrium by the reverse reaction of pyrophosphorolysis. Two closely spaced divalent metal ions (catalytic and nucleotide-binding metals) provide the scaffold for these reactions. The catalytic metal lowers the...

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PMID: 26351676
DOI: 10.1073/pnas.1511207112
PMCID: PMC4586863

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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