Article
The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2).
Journal of molecular biology - 18 Dec 1998
Stewart M, Kent H M, McCoy A J
Abstract excerpt
We report the 2.3 A resolution X-ray crystal structure of the GDP-bound form of the RanQ69L mutant that is used extensively in studies of nucleocytoplasmic transport and cell-cycle progression. When the structure of GDP-RanQ69L from monoclinic crystals with P21 symmetry was compared with the stru...
Topics
- Animals
- Binding Sites
- Biological Transport
- Carrier Proteins
- Cell Extracts
- Crystallography, X-Ray
- Cytoplasm
- GTP-Binding Proteins
- Guanosine Diphosphate
- Karyopherins
- Models, Molecular
- Mutation
- Nuclear Proteins
- Nucleocytoplasmic Transport Proteins
- Protein Conformation
- Structure-Activity Relationship
- Xenopus
- ran GTP-Binding Protein