Activities of the Porphyromonas gingivalis PrtP proteinase determined by construction of prtP-deficient mutants and expression of the gene in Bacteroides species.

Abstract excerpt

PrtP is a major cysteine proteinase of Porphyromonas gingivalis. The gene encoding this proteinase, prtP, was cloned into the Escherichia coli-Bacteroides shuttle vectors pFD288 and pFD340 and was expressed in Bacteroides cells, apparently under the control of its own promoter, when in pFD288, or...

Topics

• Animals
• Bacterial Proteins
• Bacteroides
• Cloning, Molecular
• Cysteine Endopeptidases
• Escherichia coli
• Gene Expression
• Genes, Bacterial
• Hemagglutination Tests
• In Vitro Techniques
• Mutation
• Plasmids
• Porphyromonas gingivalis
• Recombinant Proteins