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Random Mutagenesis in Porphyromonas gingivalis Enables Isolation of Mutants With Enhanced Secreted Protease Activity.

Molecular oral microbiology - 1 Jun 2026

Nakabayashi Takeru, Yuhara Satoshi, Tanaka Kosei

Abstract excerpt

Porphyromonas gingivalis is a key pathogen in periodontitis, with secreted proteases as major virulence factors. We developed a screening method to generate and identify P. gingivalis mutants with elevated protease activity. Mutations were induced using the mutagens 2,6-diaminopurine (2,6-DAP) or ethyl methanesulfonate (EMS), and the mutagenized cells were subsequently plated on casein agar. During colony growth,...

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Identifiers

PMID: 41782231
DOI: 10.1111/omi.70024
PMCID: PMC13121919

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DAP

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