Cloning and sequencing of the gene encoding a novel lysine-specific cysteine proteinase (Lys-gingipain) in Porphyromonas gingivalis: structural relationship with the arginine-specific cysteine proteinase (Arg-gingipain).

Abstract excerpt

Lys-gingipain (KGP), so termed due to its peptide cleavage specificity for lysine residues, is a cysteine proteinase produced by the Gram-negative anaerobic bacterium Porphyromonas gingivalis. Mixed oligonucleotide primers designed from the NH2-terminal sequence of the purified enzyme were used t...

Topics

• Adhesins, Bacterial
• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Cysteine Endopeptidases
• DNA Primers
• DNA, Bacterial
• Genes, Bacterial
• Gingipain Cysteine Endopeptidases
• Hemagglutinins
• Molecular Sequence Data
• Molecular Structure
• Mutation
• Polymerase Chain Reaction